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2 edition of role of the cytoplasmic domain in the localisation of the CTLA-4 and CD28 found in the catalog.

role of the cytoplasmic domain in the localisation of the CTLA-4 and CD28

Richard John Stunt

role of the cytoplasmic domain in the localisation of the CTLA-4 and CD28

by Richard John Stunt

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Published by University of Birmingham in Birmingham .
Written in English


Edition Notes

Thesis (Ph.D.) - University of Birmingham, School of Medicine, Division of Infection and Immunity, 2003.

Statementby Richard John Stunt.
The Physical Object
Pagination207 p. :
Number of Pages207
ID Numbers
Open LibraryOL21686759M

We are continually exposed to organisms that are inhaled, swallowed, or inhabit our skin and mucous membranes. Whether these organisms penetrate and cause disease is a result of both the pathogenicity of the organism (the virulence factors at its disposal) and the integrity of host defence mechanisms. The immune system is an interactive network of lymphoid organs, cells, humoral factors, and Cited by: However, it is unclear how CTLA-4 modulates NK cell responses. Further understanding of the underlying molecular mechanisms of NK cell responses will be useful in adapting and enhancing NK cell-based immunotherapy against cancer and infectious disease. To this end, we aim to elucidate the regulatory role of CTLA-4 on NK cell responses in vivo.

HEK T cell line stably expressing exogenous human CTLA-4 (CD) gene. Alternative Nomenclature. Also known as: CTLA4, CD Cell Line Characterization. Toll-like receptor 9 is a protein that in humans is encoded by the TLR9 gene. TLR9 has also been designated as CD (cluster of differentiation ). It is a member of the toll-like receptor (TLR) family. TLR9 is an important receptor expressed in immune system cells including dendritic cells, macrophages, natural killer cells, and other antigen presenting s: TLR9, CD, toll like receptor 9.

Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is a transmembrane glycoprotein that is expressed on epithelial, endothelial and immune cells. CEACAM1 is a differentiation antigen involved in the maintenance of epithelial polarity that is induced during hepatocyte differentiation and liver regeneration. CEACAM1 regulates insulin sensitivity by promoting hepatic insulin Cited by: 8. For intracellular Foxp3 and CTLA-4 staining, cultured cells were resuspended and labelled with the fluorochrome-conjugated specific mAbs against surface markers for 30 min at 4°C. The cells were then fixed and permeabilised using the Fixation/Permeabilization intracellular staining kit according to the manufacturer’s protocol (eBioscience).


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Role of the cytoplasmic domain in the localisation of the CTLA-4 and CD28 by Richard John Stunt Download PDF EPUB FB2

TAKE YOUR PARTNERS: CD28, CTLA-4 AND THEIR LIGANDS. CD28 and CTLA-4 (CD) are transmembrane protein members of the immunoglobulin gene superfamily containing a single extracellular ‘V-like’ domain.

1 – 3 Both proteins are predominantly expressed by T cells and whilst CD28 is found in substantial amounts on the cell surface of the majority of resting T cells, in contrast Cited by: CD28 and CTLA-4 are receptors on T cells that play critical roles in the initial activation and subsequent control of cellular immunity.

CD28 is expressed constitutively on T cells. It provides a major costimulatory signal upon binding to target ligands on antigen-presenting cells. How does CTLA-4 signal. CTLA-4 has a amino acid cytoplasmic tail that is devoid of intrinsic enzymatic activity and lacks a bona fide ITIM CD28, which is a surface receptor, CTLA-4 is highly endocytic, spending much of its time in intracellular vesicles ().It is constitutively present as a homodimer and does not appear to undergo conformational change following ligand by:   However, despite maximal expression being reported at 48–72 hr post‐activation, remarkably little stable surface CTLA‐4 is found, although mRNA is equivalent to that of CD 5, 6 This lower level of cell‐surface expression results from a motif in the cytoplasmic domain of CTLA‐4 that facilitates its interaction with the clathrin pit Cited by: CTLA-4 has a higher affinity for both CD80 and CD86 than does CD28 (7) (8)(9), and thus CD28 cosignaling is suppressed by CTLA-4 outcompeting CD28 for CD80 and CD86 binding.

The result is a. The results of these experiments suggest that CTLA-4 is critical for the induction of self-tolerance, and that it may have distinct signaling functions in resting and activated T cells.

In resting T cells, CTLA-4 crosslinking leads to cell-cycle arrest, whereas in activated T Cited by: CTLA-4 and T cell activation Mariette A Oosterwegel *, Rebecca J Greenwaldt Didier A Mandelbrotl, Robert B Lorsbach and Arlene H Sharpeg The past year has seen significant advances in our understanding Receptors and ligands in the of the role of cytotoxic T lymphocyte antigen 4 (CTLA-4) in B7-CD28/CTLA-4 pathway regulating T cell activation and by:   CD28 is a major T cell costimulatory receptor, the coengagement of which can prevent anergy and cell death.

The CD28 receptor is a member of a complex family of polypeptides that includes at least two receptors and two ligands. Cytotoxic lymphocyte-associated molecule-4 (CTLA-4, CD) is the second member of the CD28 receptor by: CD80 Cytoplasmic Domain Controls Localization of CD28, CTLA-4, and Protein Kinase C in the Immunological Synapse1 Su-Yi Tseng,* Mengling Liu,† and Michael L.

Dustin2* The binding of costimulatory ligand CD80 to CD28 or CTLA-4 on T cells plays an important role. Another receptor molecule, which binds to both B7 molecules and is structurally homologous to CD28, is the “cytotoxic T lymphocyte antigen 4” (CTLA-4) or CD It is composed of an IgV-type extracellular domain, a transmembrane domain, and a cytoplasmic tail.

CTLA4 is the founding member of the CD28/CTLA-4 family. Members of the CD28/CTLA-4 family either promote T cell activation (CD28 and ICOS) or inhibit T cell activation (CTLA4 and PD­1).

Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD Chromosomal Location.

Cytogenetic Location: 2q, which is the long (q) arm of chromosome 2 at position Inducible co-stimulator (ICOS) is a member of CD28/Cytotoxic T-lymphocyte Antigen-4 (CTLA-4) family and broadly expressed in activated CD4+ T cells and induced regulatory CD4+ T cells (CD4+ iTreg). ICOS-related signal pathway could be activated by the interaction between ICOS and its ligand (ICOSL).

In our previous work, we established a cost-effective system to generate a novel human allo. signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions.

CD or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation.

- auto-induces clonal expansion express CD28 and CTLA-4 - differentiates into Th0 cells during clonal expansion (activation through the IL-2R) - also express common T cell receptors and other surface proteins - Thp to Th1 and Th2 takes 7 days. In contrast to CD28, CTLA-4 restricts T cell activation, as indicated by the fact that CTLA-4 ligation inhibits IL-2 production, IL-2 receptor expression, and cell cycle progression and that CTLAdeficient mice die of a lymphoproliferative disorder within 3–4 weeks of age (reviewed in Egen et al., ).Cited by: Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells.

Linsley, P.S. et al. () J. Exp. Med. The ED 50 for this effect is μg/mL when stimulated with 1 μg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # B1)/5(3). When CD28 engages B7 molecules during T cell receptor binding, the T cell becomes activated.

CTLA-4 is highly homologous to CD28 and can also bind the B7 molecules, playing a complex role in regulating T cell function. High expression of CTLA-4 is thought to compete with CD28 and, when bound to B7, can suppress activation of T cells. CD28 and CTLA-4 are known as transmembrane glycoproteins, members of the Ig superfamily.

Their short cytoplasmic tails contain SH2- and SH3- binding domains involved in signaling events. CD28 was also reported to be constitutively expressed in T cells [. Recent reports indicate that CTLA-4 is also expressed on B cells when cultured with activated T cells, suggesting a role for CTLA-4 in the regulation of B-cell response.

Immobilized BNI3 antibody enhances T-cell proliferation induced by antibody-mediated crosslinking of CD3 and CD. The CTLA-4 protein is primarily expressed on T cells, including CD8+ cytotoxic T cells, CD4+ helper T cells, and CD4+/FoxP3+ regulatory T cells (1,2).

CTLA-4 protein competes with CD28 for B (CD80) and B (CD86) binding at the cell surface, which results in the down regulation of T cell activity (5).Category: Primary Antibodies.Protective Immunity to Filarial Parasites In Vivo1 Matthew D. Taylor,2* Anjanette Harris, Simon A.

Babayan, Odile Bain,† Abigail Culshaw, Judith E. Allen, and Rick M. Maizels The T cell coinhibitory receptor CTLA-4 has been implicated in the down-regulation of T cell function that is a quintessential feature of chronic human filarial infections.Expression of CTLA4 (CD, CD, CELIAC3, GSE, IDDM12) in placenta tissue.

Antibody staining with in immunohistochemistry.